Nov 21, 2024  
Undergraduate Course Catalog 2024-2025 
    
  Catalog Home
  Letter from the Chancellor
  The University of North Carolina System
  North Carolina Central University
  Division of Student Affairs
  Division of Extended Studies
  Administration and Faculty
  Undergraduate Admissions
  Office of Transfer Services
  National Student Exchange Program
  Scholarships and Student Aid (SSA)
  Student Expenses
  Academic Programs and Institutes
  General Education Curriculum
  Undergraduate Academic Regulations
  Transfer of Undergraduate Credit Regulation
  Credit Hour Policy
  Standard and Official Class Times
  Processes to Order an Official NCCU Transcript
  The University College
  College of Arts, Social Sciences, and Humanities
  College of Health and Science
  School of Business
  School of Education
  Course Descriptions
  APPENDIX
HELP
Undergraduate Course Catalog 2024-2025

PHRG 5120 - Advanced Biochemistry Lab (2)

Prerequisites: CHEM 3120 /CHEM 3320  - Organic Chemistry I & II and Lab,CHEM 4500 /CHEM 4520  - Biochemistry and Lab or with the consent of the instructor. This course will expand on the basic concepts covered in CHEM 4520 . Student will deepen their knowledge of and hands-on experience with cloning, bacterial expressing, enzyme purification, and assay development. The student will perform a semester-long project that involves amplification of a gene using PCR, cloning the PCR product, expressing the protein in E. coli, purifying the protein, and developing and optimizing an assay to measure enzyme activity. The purification will employ a state-of-the-art purification system from GE Healthcare, the AKTA System, which is specifically designed for protein purification. Hands-on practical experience will be gained in molecular biology, general biochemical techniques, and fundamental enzymology. Time: 3 lab hours twice a week.


Click here for the Fall 2024 Class Schedule