Aug 09, 2020  
University Undergraduate Catalog 2018-2019 
University Undergraduate Catalog 2018-2019 [ARCHIVED CATALOG]

PHRG 5120 - Advanced Biochemistry Lab (2)

Prerequisites: CHEM 3120 /CHEM 3320  - Organic Chemistry I & II and Lab,CHEM 4500 /CHEM 4520  - Biochemistry and Lab or with the consent of the instructor. This course will expand on the basic concepts covered in CHEM 4520 . Student will deepen their knowledge of and hands-on experience with cloning, bacterial expressing, enzyme purification, and assay development. The student will perform a semester-long project that involves amplification of a gene using PCR, cloning the PCR product, expressing the protein in E. coli, purifying the protein, and developing and optimizing an assay to measure enzyme activity. The purification will employ a state-of-the-art purification system from GE Healthcare, the AKTA System, which is specifically designed for protein purification. Hands-on practical experience will be gained in molecular biology, general biochemical techniques, and fundamental enzymology. Time: 3 lab hours twice a week.

Click here for the Fall 2019 Class Schedule